#C1213N 3CLPro T21I + L50F + A193P + S301P Expression Kit
Express 3CLpro with the T21I, L50F, A193P, and S301P mutations in mammalian cells including HEK293, A549, and many more. Use alongside other 3CLpro mutant proteases and our 3CLglow biosensor assays to test 3CLpro inhibitor efficacy against a panel of mutations in the protease.
3CLpro (Mpro) T21I + L50F + A193P + S301P mutation Expression Kit
Tong et al. reported a 3.6-fold increase in IC50 for GC376 using a FRET-based endpoint assay with this mutation (Tong et al. 2023). Additionally, Pfizer reported a 28.8-fold increase in EC50 to nirmatrelvir using a biochemical assay (Pfizer Laboratories 2022).
- Identify protease inhibitors that maintain efficacy against the T21I + L50F + A193P + S301P mutation
- Easy co-expression with our 3CLglow and 3CLglowUp sensors
- Cost effective screening with minimal safety requirements (BSL-1)
Materials in the Kit:
• 3CLpro T21I + L50F + A193P + S301P in BacMam
• Sodium Butyrate, 500 mM in H2O
References:
Pfizer Laboratories. (2022). (rep.). FACT SHEET FOR HEALTHCARE PROVIDERS: EMERGENCY USE AUTHORIZATION FOR PAXLOVID. Retrieved from https://labeling.pfizer.com/ShowLabeling.aspx?id=16474.
Tong, X., Keung, W., Arnold, L. D., Stevens, L. J., Pruijssers, A. J., Kook, S., Lopatin, U., Denison, M., & Kwong, A. D. (2023). Evaluation of in vitro antiviral activity of SARS-COV-2 Mpro inhibitor pomotrelvir and cross-resistance to nirmatrelvir resistance substitutions. Antimicrobial Agents and Chemotherapy, 67(11). https://doi.org/10.1128/aac.00840-23
Additional information
| Shipping | We strive for prompt delivery of each order, so we ship by FedEx 2-day service in lightweight, sturdy, and reusable/biodegradable packaging with an insulated cooler and moisture-resistant refrigerant gel packs. Shipping days are Mon-Wed so product does not sit in transit over the weekend. Web store ships to US & Canada only, contact sales for other locations. |
|---|---|
| Protocol for Use | https://montanamolecular.com/general-bacmam-transduction-protocol/ |
| MSDS | |
| Kit Volume | 5mL, 30mL |
| More Info | https://montanamolecular.com/detecting-inhibitors-of-mutant-3cl-proteases |
