Green Cell Stress Assay Kit #U0900G
Bright Green fluorescent ER Stress assay to detect response and reversal of chemical or genetic ER stress response and UPR.
A new version of this sensor is now available, product #U0921G. The sensor mechanism is the same, but the updated sensor is packaged in our new Big Sky BacMam vector and constitutively expresses the bright Montana Paintbrush RFP for improved Z’ and plate reader compatibility. Green-only measurements remain robust on plate readers and other imaging systems. Please reach out to us with any questions or for a quote for #U0900G.
The cell stress sensor is a genetically-encoded fluorescent biosensor that produces very bright green fluorescence when the cell endures endoplasmic reticulum (ER-mediated) stress or undergoes the unfolded protein response (UPR). Importantly, green fluorescent signals are nuclear localized for simple cell segmentation and image analysis. This sensor is based on the XBP1 mRNA which is spliced by the IRE1 protein during ER stress [Iwawaki et al. 2003]. Additional details may be found on the Cell Stress Assay page.
Materials in the Kit:
• Green Cell Stress Sensor BacMam
• Sodium Butyrate, 500 mM in H2O
• Thapsigargin 100 μM (control)
Additional information
| Protocol for Use | https://montanamolecular.com/cell-stress-assay-kit/cell-stress-sensor-protocol/ |
|---|---|
| Shipping | We strive for prompt delivery of each order, so we ship by FedEx 2-day service in lightweight, sturdy, and reusable/biodegradable packaging with an insulated cooler and moisture-resistant refrigerant gel packs. Shipping days are Mon-Wed so product does not sit in transit over the weekend. Web store ships to US & Canada only, contact sales for other locations. |
| MSDS | |
| More Info | |
| Scientific Publications | |
| Kit Volume | 10mL, 30mL, 60mL |
| Terms of Sale |
- G. Roy, et al. Target deconvolution of an insulin hypersecretion-inducer acting through VDAC1 with a distinct transcriptomic signature in beta-cells. bioRxiv. December 2024.
- B. Dhayalan, et al. Synthetic Studies of the Mutant Proinsulin Syndrome Demonstrate Correlation Between Folding Efficiency and Age of Diabetes Onset. International Journal of Peptide Research and Therapeutics. November 2024.
- N. Borkar, et al. Nicotine-induced ER Stress and ASM Cell Proliferation is Mediated by α7nAChR and Chaperones-RIC-3 and TMEM35. American Journal of Respiratory Cell and Molecular Biology. September 2024.
- M. Ho, et al. Molecular mechanisms involved in alcohol craving, IRF3, and endoplasmic reticulum stress: a multi-omics study. Nature Translational Psychiatry. March 2024.
- L. Scheherazade Milane. Combination Organelle Mitochondrial Endoplasmic Reticulum Therapy (COMET) for Multidrug Resistant Breast Cancer. Journal of Controlled Release. October 2023.
- S. Sanchez-Martinez, et al. Labile assembly of a tardigrade protein induces biostasis. bioRxiv. July 2023.
- N. Mohammed, et al. Mechanisms of cell death induced by hexabromocyclododecane (HBCD) involves apoptosis, autophagy, and ER stress. Journal of Biochemical and Molecular Toxicology. June 2023.
- K. Harlen, et al. Live-Cell Assays for Cell Stress Responses Reveal New Patterns of Cell Signaling Caused by Mutations in Rhodopsin, α-Synuclein and TDP-43 Frontiers Cellular Neuroscience. December 2019.
- K. Harlen, et al. Live-cell biosensors reveal novel insight into neurotoxic and cardiotoxic compound mediated cellular stress response. Journal of Pharmacological and Toxicological Methods. October 2019.
BioTek Application Note
Explore Thapsigargin-induced Cellular Stress Response and Inhibition of Gq-dependent Calcium Signaling
with Lionheart™ FX Automated Microscope
