FPX Biosensors for Live Cell Imaging Published
It is always a pleasure to collaborate with our neighbors at University of Alberta!
As the Campbell Lab so succinctly summarized this work: “First there was FRET, now there is FPX!” Many thanks to Yidan Ding, Robert Campbell and all of our co-authors for the opportunity to collaborate on developing this remarkable new approach to making genetically-encoded biosensors for live cell imaging.
Check out the back story behind how Robert Campbell and his team came up with the dimerization dependent fluorescent proteins that are the basis of FPX Biosensors, and how Montana Molecular and others were able to leverage this approach to build a wide variety of biosensors that detect very diverse signaling processes in living cells.
Interested in using FPX biosensors in your lab?
Visit AddGene for FPX Biosensor plasmids.
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Yidan Ding, Jiao Li, Jhon Ralph Enterina, Yi Shen, Issan Zhang, Paul H. Tewson, Gary C. H. Mo, Jin Zhang, Anne Marie Quinn, Thomas E. Hughes, Dusica Maysinger,Spencer C. Alford, Yan Zhang, Robert E. Campbell (2015) Ratiometric biosensors based on dimerization dependent fluorescent protein exchange. Nature Methods, doi:10.1038/nmeth.3261.
Ratiometric biosensors based on dimerization-dependent fluorescent protein exchange