#U0266G AKAP Targeted cADDis cAMP Sensor – AKAP12

U0266G is a fusion of the complete AKAP12 protein and the cADDis biosensor. In HEK293 cells the fluorescence is targeted to the plasma membrane and this sensor responds nicely to isoproterenol stimulation of endogenous β adrenergic receptors.

Measure cAMP signaling in real time, in the living cells of your choice, with the AKAP12-targeted cADDis cAMP sensor. Increase in fluorescence indicates increase in cAMP presence. cADDis is genetically-encoded and packaged in a BacMam viral vector for easy transduction into a wide variety of cell types, including primary and iPSC-derived cell lines. Simply add cADDis to your cells and measure robust signals the very next day on standard fluorescence microscopes and automated plate readers.

Materials in the Kit:

• Green AKAP12-targeted cADDis cAMP sensor BacMam
• Sodium Butyrate, 500 mM in H₂O
• β2 Adrenergic Receptor BacMam
• Isoproterenol 10 mM, in 10mM HCl

AKAP12

AKAP12 (also known as Gravin or AKAP250) is largely localized to the plasma membrane, though the addition of phorbol esters, to stimulate protein kinase C, can cause a redistribution of AKAP12 intracellular membrane compartments. (Yan et al., 2009) There is also evidence that AKAP12 is involved in hippocampal plasticity (Havekes et al., 2012) and that it coordinates the PKA and phosphatase activity at the β adrenergic receptor in the heart. (Havekes et al., 2012; McConnell et al., 2016)