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#C1215N 3CLPro T21I + C160F + A173V + V186A + T304I Expression Kit


Express 3CLpro with the T21I, C160F, A173V, V186A, and T304I mutations in mammalian cells including HEK293, A549, and many more. Use alongside other 3CLpro mutant proteases and our 3CLglow biosensor assays to test3CLpro inhibitor efficacy against a panel of mutations in the protease.



3CLpro (Mpro) T21I + C160F + A173V + V186A + T304I mutation Expression Kit

The T21I + C160F + A173V + V186A + T304I mutation combination has been detected by... and resistance to nirmatrelvir was confimed by.... Text needed - same as the blog post.

  • Identify protease inhibitors that maintain efficacy against the T21I + C160F + A173V + V186A + T304I mutation
  • Easy co-expression with our 3CLglow and 3CLglowUp sensors
  • Cost effective screening with minimal safety requirements (BSL-1)

Materials in the Kit:

• 3CLpro T21I + C160F + A173V + V186A + T304I in BacMam
• Sodium Butyrate, 500 mM in H2O


Tong et al. reported a 4.9-fold increase in IC50 for GC376 using a FRET-based endpoint assay with this mutation (Tong et al. 2023). Pfizer reported a 28.5-fold increase in EC50 to nirmatrelvir using a biochemical assay (Pfizer Laboratories 2022). 


Additional information


We strive for prompt delivery of each order, so we ship by FedEx 2-day service in lightweight, sturdy, and reusable/biodegradable packaging with an insulated cooler and moisture-resistant refrigerant gel packs. Shipping days are Mon-Wed so product does not sit in transit over the weekend. Web store ships to US & Canada only, contact sales for other locations.

Protocol for Use


Kit Volume

5mL, 30mL

More Info