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#C1203N 3CLPro E166G Expression Kit


Express 3CLpro with the E166G mutation in mammalian cells including HEK293, A549, and many more. Use alongside other 3CLpro mutant proteases and our 3CLglow biosensor assays to test 3CLpro inhibitor efficacy against a panel of mutations in the protease.



3CLpro (Mpro) E166G mutation Expression Kit

The E166G mutation seems to be associated with reduced enzymatic activity and increased Ki values for nirmatrelvir, according to Hu et al. and Tan et al. (Tan et al. 2023; Hu et al. 2023) In both studies, E166G exhibited a 7.4-fold reduction in enzymatic activity, and a 16.4-fold increase in the Ki value for nirmatrelvir (Tan et al. 2023; Hu et al. 2023). Additionally, this mutation has been detected in patients and documented in the GISAID database (Shu and McCauley 2017).

  • Identify protease inhibitors that maintain efficacy against the E166V mutation
  • Easy co-expression with our 3CLglow and 3CLglowUp sensors
  • Cost effective screening with minimal safety requirements (BSL-1)

Materials in the Kit:

• 3CLpro E166G in BacMam
• Sodium Butyrate, 500 mM in H2O


Hu, Y., Lewandowski, E. M., Tan, H., Zhang, X., Morgan, R. T., Zhang, X., Jacobs, L. M., Butler, S. G., Gongora, M. V., Choy, J., Deng, X., Chen, Y., & Wang, J. (2023). Naturally occurring mutations of SARS-COV-2 main protease confer drug resistance to Nirmatrelvir. ACS Central Science, 9(8), 1658–1669.

Shu, Y., & McCauley, J. (2017). GISAID: Global initiative on sharing all influenza data – from vision to reality. Eurosurveillance, 22(13).

Tan, B., Joyce, R., Tan, H., Hu, Y., & Wang, J. (2023). SARS-COV-2 main protease drug design, Assay Development, and Drug Resistance Studies. Accounts of Chemical Research, 56(2), 157–168.

Additional information


We strive for prompt delivery of each order, so we ship by FedEx 2-day service in lightweight, sturdy, and reusable/biodegradable packaging with an insulated cooler and moisture-resistant refrigerant gel packs. Shipping days are Mon-Wed so product does not sit in transit over the weekend. Web store ships to US & Canada only, contact sales for other locations.

Protocol for Use


Kit Volume

5mL, 30mL

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