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#C1110G Pseudo SARS-CoV-2 Green Reporter

BacMam virus pseudotyped with SARS-CoV-2 Spike protein. A pseudovirus that will produce green fluorescent nuclei upon successful entry, into host cells expressing ACE2

SARS-CoV-2 assays and reagents

 

BacMam vector pseudotyped with SARS-CoV-2 spike protein:

Pseudo SARS-CoV-2 is a BacMam vector pseudotyped with SARS-CoV-2 spike protein. Pseudovirus enters the host cell through interactions with the ACE2 protein and TMPRSS2 protein, then delivers a genetically-encoded, fluorescent reporter that expresses bright green fluorescence in the host cell nucleus. When viral entry is successfully blocked, the host cell nucleus is dark. Safe and robust tools to study SARS-CoV-2 viral entry are now available to empower discovery of compounds or antibodies that can block host infection.

  • Detect host-pathogen interaction in living cells
  • Identify therapeutic drugs that block ACE2-mediated viral entry
  • Screen for neutralizing antibodies to SARS-CoV-2 spike D614G
  • Cost effective screening with minimal safety requirements (BSL-1)

Pseudo SARS-CoV-2 BacMam is pseudotyped with the original variant of the SARS-CoV-2 spike protein formed during BacMam production. Spike decorates the surface of the pseudovirus, facilitating entry into cells expressing ACE2 and TMPRSS2. BacMam is high titer and more cost effective to use than lentivirus.

 


Materials in the Kit:

• Pseudo SARS CoV-2 Green NLS

• ‘Bald’ BacMam virus without VSVG or spike is a control for the pseudo-SARS-CoV-2 virus

• Sodium Butyrate, 500 mM in H2O

• Detailed protocol

 

Product Info & Protocols

Protocol for Use

https://montanamolecular.com/fluorescent-pseudo-sars-cov-2-reporter-protocol/

Shipping

We strive for prompt delivery of each order, so we ship by FedEx 2-day service in lightweight, sturdy, and reusable packaging with an insulated cooler and moisture-resistant refrigerant gel packs. Shipping days are Mon-Wed so product does not sit in transit over the weekend. Web store ships to US & Canada only, contact sales for other locations. Purchases over $600 receive free shipping.

MSDS

https://montanamolecular.com/wp-content/uploads/2020/05/Pseudo-SARS-CoV-2-MSDS-PDF.pdf

Kit Volume

5mL, 30mL, 60mL

More Info

https://montanamolecular.com/covid-19-drug-discovery/

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