Pseudo SARS-CoV-2 D614G Mutation Green Reporter #C1120G
Baculovirus pseudotyped with SARS-CoV-2 D614G mutated spike protein that binds with ACE2 and expresses green fluorescent protein at the nucleus 24 hours after viral entry.
SARS-CoV-2 assays and reagents
Baculovirus vector pseudotyped with D614G mutated SARS-CoV-2 spike protein:
D614G Pseudo SARS-CoV-2 is a BSL-1 baculovirus pseudotyped with D614G spike isolate. Pseudovirus enters the host cell through interactions with ACE2 and other host proteins, then delivers a reporter that expresses bright green fluorescence in the host cell nucleus. When viral entry is successfully blocked, the host fluorescence is decreased.
D614G PseudoSARS-CoV-2 is pseudotyped with the D614G variant of the SARS-CoV-2 spike protein. This European isolate spike protein decorates the surface of the pseudovirus, facilitating entry into cells expressing ACE2.
- Detect host-pathogen interaction in living cells.
- Identify therapeutic drugs that block ACE2-mediated viral entry.
- Cost effective screening with minimal safety requirements (BSL-2).
Materials in the Kit:
• D614G Pseudo SARS CoV-2 Green NLS
• Sodium Butyrate, 500 mM in H2O
• Detailed protocol
Product Info & Protocols
|Protocol for Use|
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5mL, 30mL, 60mL