IRE1-XBP1 Cell Stress Assay #U0921G
Detect stress responses on a plate reader with the bright IRE1-XBP1 ratiometric cell stress sensor. CMV promoter.
This is an updated version of product #U0901G. It is packaged in our new Big Sky BacMam vector and constitutively expresses the brighter Montana Paintbrush RFP for improved plate reader compatibility.
This sensor is based on the XBP1 mRNA which is spliced by the IRE1 protein during ER stress. A red fluorescent protein is constitutively expressed while the sensor produces very bright green fluorescence when the cell endures endoplasmic reticulum (ER-mediated) stress or undergoes the unfolded protein response (UPR). Measure stress using just the green fluorescence or take a ratio of the green and red channels. Additionally, both the red and green fluorescent proteins are nuclear localized for simple cell segmentation and image analysis. More details may be found on the Cell Stress Assay page.
Materials in the Kit:
• IRE1-XBP1 Ratiometric Cell Stress sensor BacMam
• Sodium Butyrate, 500 mM in H2O
• Thapsigargin (positive control)
Additional information
| Protocol for Use | Coming Soon |
|---|---|
| Shipping | We strive for prompt delivery of each order, so we ship by FedEx 2-day service in lightweight, sturdy, and reusable/biodegradable packaging with an insulated cooler and moisture-resistant refrigerant gel packs. Shipping days are Mon-Wed so product does not sit in transit over the weekend. Web store ships to US & Canada only, contact sales for other locations. |
| MSDS | |
| More Info | |
| Scientific Publications | |
| Kit Volume | 5mL |
| Terms of Sale |
- M. Ho, et al. Molecular mechanisms involved in alcohol craving, IRF3, and endoplasmic reticulum stress: a multi-omics study. Nature Translational Psychiatry. March 2024.
- L. Scheherazade Milane. Combination Organelle Mitochondrial Endoplasmic Reticulum Therapy (COMET) for Multidrug Resistant Breast Cancer. Journal of Controlled Release. October 2023.
- S. Sanchez-Martinez, et al. Labile assembly of a tardigrade protein induces biostasis. bioRxiv. July 2023.
- N. Mohammed, et al. Mechanisms of cell death induced by hexabromocyclododecane (HBCD) involves apoptosis, autophagy, and ER stress. Journal of Biochemical and Molecular Toxicology. June 2023.
- K. Harlen, et al. Live-Cell Assays for Cell Stress Responses Reveal New Patterns of Cell Signaling Caused by Mutations in Rhodopsin, α-Synuclein and TDP-43 Frontiers Cellular Neuroscience. December 2019.
- K. Harlen, et al. Live-cell biosensors reveal novel insight into neurotoxic and cardiotoxic compound mediated cellular stress response. Journal of Pharmacological and Toxicological Methods. October 2019.
BioTek Application Note
Explore Thapsigargin-induced Cellular Stress Response and Inhibition of Gq-dependent Calcium Signaling
with Lionheart™ FX Automated Microscope
