Nanog – Yamanaka Factors – #Y0070N

Nanog is occasionally added to the mix of transcription factors used to create iPSC cells. Transcription regulator involved in inner cell mass and embryonic stem (ES) cells proliferation and self-renewal. Imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages.  The Reference sequence is NM_001297698.2.

Advantages to using BacMam for Yamanaka Factor expression:

• Adjust the expression levels of the factors by simply adjusting the amount of the BacMam you add to the culture.
• Adjust the relative amounts of  transcription factor expression  by adjusting the ratio of the different amounts of the different BacMam viruses you add.
• Transduce the cells multiple times, over multiple days, to produce prolonged expression.
• The BacMam vector produces transient, integration free expression.
• BacMam vectors are shelf stable in the refrigerator for over a year.
• BacMam vectors are cost effective.  Each of our products contains 10 mls of virus at 2 * 10e10 viral genes per ml, exceeding competitors Lentivirus products by orders of magnitude.