Red GECO Ca2+ Assay – Big Sky (U0650R)
Price range: $895.00 through $2,995.00
Red fluorescent calcium assay for detecting real-time calcium signaling on fluorescence plate readers and imaging systems. Standard stock comes with CMV promoter.
This is an updated version of product #U0600R, the difference being that the R-GECO biosensor is packaged in Montana Molecular's Big Sky BacMam vector.
The fluorescent, genetically-encoded Ca2+ indicator (GECO) was developed by Robert Campbell’s lab at University of Alberta. R-GECO can be paired with green fluorescent cADDis cAMP sensors or DAG sensors to simultaneously detect both Ca2+ and cyclic AMP or diacylglycerol in living cells. The BacMam delivery system enables consistent and controllable expression in a wide variety of cell types including iPSC-derived cardiomyocytes and neurons. More details on this tool can be found on the Calcium Assays page.
Materials in the Kit:
• R-GECO Ca2+ Sensor Big Sky BacMam
• Sodium Butyrate, 500 mM in H2O
• Z0200G M1 Muscarinic acetylcholine Receptor BacMam with nuclear green label
• Carbachol 25 mM in H2O
Additional information
| Protocol for Use and Kit Insert | https://montanamolecular.com/calcium-assay-kit-geco/red-geco-ca2-protocol/ |
|---|---|
| Shipping | We strive for prompt delivery of each order, so we ship by FedEx 2-day service in lightweight, sturdy, and reusable/biodegradable packaging with an insulated cooler and moisture-resistant refrigerant gel packs. Shipping days are Mon-Wed so product does not sit in transit over the weekend. Web store ships to US & Canada only, contact sales for other locations. |
| SDS | |
| More Info | |
| Scientific Publications | |
| Kit Volume | 15mL, 5mL, 30mL |
| Terms of Sale |
GPCR Assay References
Borealis Arrestin Assay References
- HD Tran, et al. Modelling G protein-biased agonism using GLP-1 receptor C-terminal mutations. Molecular Metabolism. January 2026.
- Y. Manchada, et al. Glucagon-like peptide-1 receptor C-terminal tail phosphorylation determines signalling responses from pancreatic β-cells. bioRxiv. November 2025.
- A. Ippolito, et al. Evidence that 5-HT2A receptor signalling efficacy and not biased agonism differentiates serotonergic psychedelic from non-psychedelic drugs. British Journal of Pharmacology. June 2025.
- E. Billard, et al. Pharmacological characterization of cannabidiol as a negative allosteric modulator of the 5-HT2A receptor. Cellular Signaling. January 2025.
- I. Davies, et al. Chronic GIPR agonism results in pancreatic islet GIPR functional desensitisation. Molecular Metabolism. January 2025.
- A. Ippolito, et al. Increased 5-HT2A receptor signalling efficacy differentiates serotonergic psychedelics from non-psychedelics. bioRxiv. June 2024.
- H. Schiff, et al. β-arrestin-biased proteinase-activated receptor-2 antagonist C781 limits allergen-induced airway hyperresponsiveness and inflammation. British Journal of Pharmacology. June 2022.
cADDis cAMP Assay References
- N. Annamdevula, et al. Combined excitation scan-based hyperspectral imaging and digital deconvolution for the assessment of the kinetic and spatial distributions of cAMP signals. SPIE. March 2026.
- G. Toro-Tapia, et al. Ciliary cAMP regulates Shh signal interpretation to drive polarisation of differentiating neurons. bioRxiv. March 2026.
- B. Wysolmerski, et al. Conformational biosensors delineate endosomal G protein regulation by GPCRs. Nature Communications. February 2026.
- L. El Eid, et al. In vivo functional profiling and structural characterization of the human GLP1R A316T variant. Science Advances. February 2026.
- E. Blythe, et al. GPCR-selective effects of endocytosis on cellular signaling through the cAMP / PKA cascade. Journal of Biological Chemistry. February 2026.
- H. Hamzeh, et al. Deciphering Rapid Cell Signaling and Control of Cell Motility by Reverse Opto-Chemical Engineering. PRX Life. February 2026.
- HD Tran, et al. Modelling G protein-biased agonism using GLP-1 receptor C-terminal mutations. Molecular Metabolism. January 2026.
- R. Gao, et al. Antecedent hypoglycaemia impairs glucagon secretion by enhancing somatostatin-mediated negative feedback control. Nature Metabolism. January 2026.
- I. Davies, et al. A metabolic comparison of GIPR agonism versus GIPR antagonism in male mice. Diabetes, Obesity, and Metabolism. January 2026.
- G. Austin, et al. GLP-1R associates with VAPB and SPHKAP at ERMCSs to regulate β-cell mitochondrial remodelling and function. Nature Communications. December 2025.
- S. Chen, et al. Spatially diffuse cAMP signalling with oppositely biased GLP-1 receptor agonists in β-cells despite differences in receptor localisation. Molecular Metabolism. December 2025.
- M. Dickerson, et al. Glycolytic activation of β-cell Na+/K+-ATPases containing β1-subunits accelerates Na+ extrusion, prolonging the duration of Ca2+ oscillations but decreasing insulin secretion. Molecular Metabolism. December 2025.
- S. Orfanos, et al. The differential effects of cAMP mobilizing agents on TGF-β-induced extracellular matrix in human lung-derived fibroblasts: insights into therapeutic targets for lung fibrosis. Respiratory Research. November 2025.
- Y. Manchada, et al. Glucagon-like peptide-1 receptor C-terminal tail phosphorylation determines signalling responses from pancreatic β-cells. bioRxiv. November 2025.
- C. Metrick, et al. GPR17 structure and agonism with small molecules and oxysterols. bioRxiv. November 2025.
- S. Zervou, et al. Effect of whole-body creatine-deficiency on secretion of insulin and glucagon from isolated mouse islets. SSRN. October 2025.
- Mark Lee & Keren Hilgendorf. Prostaglandin E2 inhibits adipogenesis through the cilia-dependent activation of ROCK2. Journal of Cell Science. September 2025.
- R. Nassini, et al. Targeting prostaglandin E2 receptor 2 in Schwann cells inhibits inflammatory pain but not inflammation. Nature Communications. September 2025.
- Tatsuru Togo. Disruption of the cell membrane triggers Ca2+ mobilization using P2Y receptors and subsequent cAMP synthesis in cells surrounding wounded MDCK cells. Biochemical and Biophysical Research Communications. September 2025.
- C. Sturaro, et al. Activation of peripheral NOP receptors reduces periorbital mechanical allodynia evoked by CGRP in mice. British Journal of Pharmacology. August 2025.
- T. Cacciottolo, et al. Glucagon Receptor Deficiency Causes Early-Onset Hepatic Steatosis. Diabetes. August 2025.
- J. Chadwick, et al. Dietary-dependent sensitization of neuronal leptin signaling promotes neural repair after injury via cAMP and gene transcription. Neuron. August 2025.
- K. Berger, et al. Design of a peripherally biased NPSR1 antagonist for neuropeptide S induced inflammation. Bioorganic & Medicinal Chemistry Letters. August 2025.
- C. Gao, et al. Semaglutide drives weight loss through cAMP-dependent mechanisms in GLP1R-expressing hindbrain neurons. bioRxiv. August 2025.
- Y. Manchanda, et al. Glycaemic and bodyweight effects of GIPR coding variation reflect differences in both surface expression and intrinsic functional impairment. medRxiv. August 2025.
- S. Orfanos, et al. The differential effects of cAMP mobilizing agents on TGF-β-induced extracellular matrix in human lung-derived fibroblasts: Insights into therapeutic targets for lung fibrosis. Research Square. August 2025.
- N. Saito, et al. Activation of G protein-coupled parathyroid hormone receptors in rat incisor odontoblasts promotes mineralization via cyclic adenosine monophosphate, not Ca2+ signalling: In vitro study. International Endodontic Journal. July 2025.
- M. Merz, et al. Downstream Signaling of Muscarinic M4 Receptors Is Regulated by Receptor Density and Cellular Environment. Pharmacology Research & Perspectives. May 2025.
- B. Wysolmerski, et al. Conformational biosensors delineate endosomal G protein regulation by GPCRs. bioRxiv. May 2025.
- Paul G. DeCaen & Louise F. Kimura. Methods to Assess Neuronal Primary Cilia Electrochemical Signaling. Journal of Cellular Physiology. April 2025.
- A. Oqua, et al. Molecular mapping and functional validation of GLP-1R cholesterol binding sites in pancreatic beta cells. eLife. April 2025. (bioRxiv)
DAG Assay References
- E. Lukovic, et al. Design and Evaluation of Novel Ginger 6-Shogaol-Inspired Phospholipase C Inhibitors to Enhance β-Agonist-Induced Relaxation in Human Airway Smooth Muscle. Journal of Medicinal Chemistry. June 2025.
- E. Billard, et al. Pharmacological characterization of cannabidiol as a negative allosteric modulator of the 5-HT2A receptor. Cellular Signaling. January 2025.
- X. Chen, et al. Roles for PKC signaling in chromaffin cell exocytosis. Biophysical Journal. December 2024.
- A. Hamilton, et al. Nicotinic signaling stimulates glucagon secretion in mouse and human pancreatic α-cells. Diabetes. October 2024.
- X. Chen, et al. A PACAP-activated network for secretion requires coordination of Ca2+ influx and Ca2+ mobilization. Molecular Biology of the Cell. May 2024. (bioRxiv)
- S. Datta, et al. APOL1-mediated monovalent cation transport contributes to APOL1-mediated podocytopathy in kidney disease. The Journal of Clinical Investigation. January 2024. (Supplemental Data)
- Z. Miller, et al. Lidocaine induces apoptosis in head and neck squamous cell carcinoma through activation of bitter taste receptor T2R14. Cell Reports. November 2023. (bioRxiv)
- N. Zaïmia, et al. GLP-1 and GIP receptors signal through distinct β-arrestin 2-dependent pathways to regulate pancreatic β cell function. Cell Reports. October 2023.
- G. Sanchez, et al. Coincident Regulation of PLCβ Signaling by Gq-Coupled and μ-Opioid Receptors Opposes Opioid-Mediated Antinociception. Molecular Pharmacology. December 2022.
- Z. Miller, et al. Lidocaine Induces Apoptosis in Head and Neck Squamous Cell Carcinoma Cells Through Activation of Bitter Taste Receptor T2R14. bioRxiv. November 2022.
- M. Doepner, et al. Endogenous DOPA inhibits melanoma through suppression of CHRM1 signaling. Science Advances. September 2022.
PIP2 Assay References
- V. Chandar, et al. Intercellular contact is sufficient to drive Fibroblast to Myofibroblast transitions. arXiv. March 2025.
- L. Brueggemann, et al. Structural Determinants of Kv7.5 Potassium Channels that Confer Changes in Phosphatidylinositol 4,5-Biphosphate (PIP2) Affinity and Signaling Sensitivity in Smooth Muscle Cells. Molecular Pharmacology. March 2020.
- L.Liu, et al. Gαq sensitizes TRPM8 to inhibition by PI(4,5)P2 depletion upon receptor activation. J.Neurosci. May 2019.
- ,  and  Teaching an Old Drug New Tricks: Agonism, Antagonism, and Biased Signaling of Pilocarpine through M3 Muscarinic Acetylcholine Receptor Molecular Pharmacology Nov. 2018
GECO Calcium Assay References
- X. Chen, et al. Roles for PKC signaling in chromaffin cell exocytosis. Biophysical Journal. December 2024.
- X. Chen, et al. In vitro and in vivo inhibition of the host TRPC4 channel attenuates Zika virus infection. EMBO Molecular Medicine. July 2024.
- X. Chen, et al. A PACAP-activated network for secretion requires coordination of Ca2+ influx and Ca2+ mobilization. Molecular Biology of the Cell. May 2024. (bioRxiv)
- C. Amos, et al. Membrane lipids couple synaptotagmin to SNARE-mediated granule fusion in insulin-secreting cells. Molecular Biology of the Cell. December 2023.
- J. Wu, et al. Interaction Between HCN and Slack Channels Regulates mPFC Pyramidal Cell Excitability and Working Memory. bioRxiv. March 2023.
- M. Thomas, et al. Optically activated, customizable, excitable cells. PLOS One. December 2020.
- L. Liu, et al. Diacylglycerol kinases regulate TRPV1 channel activity. Journal of Biological Chemistry. April 2020.
- S. Hoare, et al. A kinetic method for measuring agonist efficacy and ligand bias using high resolution biosensors and a kinetic data analysis framework. Nature Scientific Reports Feb 2020.
- K. Harlen, et al. Live-Cell Assays for Cell Stress Responses Reveal New Patterns of Cell Signaling Caused by Mutations in Rhodopsin, α-Synuclein and TDP-43 Front. Cell. Neurosci.,December 2019
