#U0262G AKAP Targeted cADDis cAMP Sensor – AKAP18b

U0262G is a fusion of the complete AKAP18b protein to the cADDis biosensor. In HEK293T cells the fluorescence is targeted to the plasma membrane.  This sensor responds nicely to isoproterenol stimulation of endogenous β adrenergic receptors of HEK293 cells.

Measure cAMP signaling in real time, in the living cells of your choice, with the AKAP18b-targeted cADDis cAMP sensor. Increase in fluorescence indicates increase in cAMP presence. cADDis is genetically-encoded and packaged in Montana Molecular's Big Sky BacMam vector for consistent, titratable transduction into a wide variety of cell types, including primary and iPSC-derived cell lines. Simply add cADDis to your cells and measure robust signals the very next day.

Materials in the Kit:

• Green AKAP18b-targeted cADDis cAMP sensor BacMam
• Sodium Butyrate, 500 mM in H₂O
• β2 Adrenergic Receptor BacMam with nuclear red label
• Isoproterenol 10 mM, in 10mM HCl

AKAP18b (Also known as AKAP7)

AKAP 18b is identical in sequence to AKAP 18a except that it contains an additional 23 amino acid insert that directs it to the apical surface in polarized endothelial cells. (Trotter et al., 1999).  Fused to the cADDis sensor, the fluorescence is targeted to the plasma membrane of HEK 293 cells.  This sensor, expressed in HEK 293 cells, responds nicely to isoproterenol stimulation of endogenous β adrenergic receptors.