Montana Molecular News
Expression in iPSC Derived Neurons
Live Cell Painting of iPSC-derived neurons BacMam delivers live cell painting tools to BrainXell’s iPSC cortical glutamatergic neurons Optimal expression does not involve HDAC inhibitors A synapsin promoter driven reporter reveals neuronal...
Painting the Omegasome
Painting the OmegasomeVisualize proteostasis and autophagy processes in living cells with a fluorescent probeGreen OmegaPaint is a new addition to our suite of Cell Painting tools, combining the bright mNeonGreen fluorescent protein with a portion of the DFCP1 protein...
Express RAMPs with BacMam
Express RAMPs with BacMamBacMam-packaged RAMP1, RAMP2, and RAMP3 are now available from Montana Molecular! Receptor Activity-Modifying Proteins (RAMPs) can regulate the function and specificity of G-protein-coupled receptors - influencing their trafficking, ligand...
Introducing Big Sky BacMam
Introducing Big Sky BacMamThe R&D team at Montana Molecular has been hard at work improving our BacMam vector! We're excited to release Big Sky BacMam - a vector which, in our hands, yields greatly improved transduction efficiency in HEK and CHO cells. In HEK293T...
Detecting inhibitors of mutant 3CL proteases
Find your next-generation SARS-CoV-2 inhibitor using mutant 3CL proteasesThe SARS-CoV-2 protease is crucial to virus replication. If the main protease, known as 3CLpro can be blocked, the virus can be stopped. The commonly prescribed Paxlovid contains a 3CLpro...
Montana Paintbrush
Montana PaintbrushMontana Paintbrush (Paintbrush, MT Paintbrush) is a bright, rapidly folding red fluorescent protein developed at Montana Molecular. It is nearly twice as bright as mCherry 24 hours after transduction as seen in Figure 1 below. Figure 1: Fluorescence...
Next-generation drugs for the evolving SARS-CoV-2 virus
Next-generation drugs for the evolving SARS-CoV-2 virusThere is increasing evidence that new variants of SARS-CoV-2 are appearing that cannot be treated with Paxlovid. This means that the next generation of antivirals will soon be needed to reduce the severity of...
New AKAP-targeted cADDis cAMP Sensors
New AKAP-targeted cADDis SensorsMost cAMP measurements are global measurements of total cellular cAMP. However when biosensors are targeted to different subcellular domains, they often reveal more nuanced views of very localized cAMP signaling. There are many...
Form and Function: consequences of subcellular cAMP signaling revealed
In a recent paper, Isabella Cattani-Cavalieri and her colleagues in Ren Ostrom’s laboratory found a new, powerful way to examine the functional consequences of different cAMP signalsomes (Cattani-Cavalieri et al., 2023). There has long been a suspicion that there are...
A breathtaking model for the cellular detection of hypoxia
In a recent paper, Peng and colleagues (Peng et al. 2023) use a variety of new approaches, and fresh ideas, to create a molecular model for how the cells of the carotid body sense the oxygen levels in our blood and use this to control our breathing. Surprisingly, key...
