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#C1206N 3CLPro E166A + L167F Expression Kit


Express 3CLpro with the E166A and L167F mutations in mammalian cells including HEK293, A549, and many more. Use alongside other 3CLpro mutant proteases and our 3CLglow biosensor assays to test 3CLpro inhibitor efficacy against a panel of mutations in the protease.



3CLpro (Mpro) E166A + L167F mutation Expression Kit

In VeroE6 cells, employing a FRET-based assay with a presence of .5μM CP 100356, the E166A, L167F mutation had a 10-fold change in the EC50 (Jochmans et al. 2023). This mutation is a component of the in vitro emergent mutation L50F/E166A/L167F (Jochmans et al. 2023). 

  • Identify protease inhibitors that maintain efficacy against the E166A + L167F mutation
  • Easy co-expression with our 3CLglow and 3CLglowUp sensors
  • Cost effective screening with minimal safety requirements (BSL-1)

Materials in the Kit:

• 3CLpro E166A + L167F in BacMam
• Sodium Butyrate, 500 mM in H2O


Jochmans, D., Liu, C., Donckers, K., Stoycheva, A., Boland, S., Stevens, S. K., De Vita, C., Vanmechelen, B., Maes, P., Trüeb, B., Ebert, N., Thiel, V., De Jonghe, S., Vangeel, L., Bardiot, D., Jekle, A., Blatt, L. M., Beigelman, L., Symons, J. A., … Vandyck, K. (2023). The substitutions L50F, E166A, and L167F in SARS-COV-2 3clpro are selected by a protease inhibitor in vitro and confer resistance to Nirmatrelvir. mBio, 14(1).

Additional information


We strive for prompt delivery of each order, so we ship by FedEx 2-day service in lightweight, sturdy, and reusable/biodegradable packaging with an insulated cooler and moisture-resistant refrigerant gel packs. Shipping days are Mon-Wed so product does not sit in transit over the weekend. Web store ships to US & Canada only, contact sales for other locations.

Protocol for Use


Kit Volume

5mL, 30mL

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