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#X0250G Green Gi cADDis cAMP Assay Kit (Big Sky)

$795.00$2,895.00

Screen Gi-coupled GPCRs to monitor inhibition of cAMP production on fluorescence plate readers or imaging systems. Standard stock comes with CMV promoter. Other promoters are available, please inquire!


 

 

This is an updated version of product #X0200G, the difference being that the cADDis cAMP biosensor is packaged in Montana Molecular's Big Sky BacMam vector.

A simple and robust assay for Gi-coupled receptor signaling without the need for co-factors or cell lysis. cADDis is a genetically-encoded biosensor for cAMP that is packaged in a BacMam viral vector for easy transduction into a wide variety of cell types, including primary and iPSC-derived cell lines. Measure robust signals 24 hours post transduction on standard fluorescence microscopes and automated plate readers. See additional details on the cADDis cAMP assays page.


Materials in the Kit:

• Green Downward cADDis BacMam

• Sodium Butyrate, 500 mM in H2O

hD2 Receptor BacMam

• Quinpirole Hydrochloride 2 mM, in sterile H2O

Additional information

Protocol for Use

https://montanamolecular.com/live-cell-camp-assay-caddis/green-gi-caddis-camp-protocol/

Shipping

We strive for prompt delivery of each order, so we ship by FedEx 2-day service in lightweight, sturdy, and reusable/biodegradable packaging with an insulated cooler and moisture-resistant refrigerant gel packs. Shipping days are Mon-Wed so product does not sit in transit over the weekend. Web store ships to US & Canada only, contact sales for other locations.

MSDS

https://montanamolecular.com/msds-for-caddis-assays/

Scientific Publications

https://montanamolecular.com/scientific-publications//

More Info

https://montanamolecular.com/live-cell-camp-assay-caddis

Kit Volume

15mL, 5mL, 30mL

Terms of Sale

https://montanamolecular.com/terms-of-sale/

GPCR Assay References
cADDis cAMP Assay References