#C1123G Pseudo SARS-CoV-2 Spike Delta Variant Green Reporter
$695.00 – $3,895.00
Baculovirus pseudotyped with SARS-CoV-2 Delta Variant Spike protein, also known as B.1.617.2. The pseudovirus will produce green fluorescent nuclei upon successful entry, into host cells expressing ACE2.
SARS-CoV-2 assays and reagents
Baculovirus pseudotyped with a SARS-CoV-2 Delta Variant (B.1.617.2) spike protein:
Pseudo SARS-CoV-2 is a BSL-1 baculovirus pseudotyped with SARS-CoV-2 spike protein. Pseudovirus enters the host cell through interactions with ACE2 and other host proteins, then delivers a reporter that expresses bright green fluorescence in the host cell nucleus. When viral entry is successfully blocked, the host fluorescence is decreased.
- Detect host-pathogen interaction in living cells
- Identify therapeutic drugs that block viral entry
- Cost effective screening with minimal safety requirements
This Pseudo SARS-CoV-2 Delta version is pseudotyped with a SARS-CoV-2 Delta Variant spike protein, also known as B.1.617.2. The mutations in the Delta Variant are T19R, V70F, T95I, G142D, E156-, F157-, R158G, A222V, W258L, K417N, L452R, T478K, D614G, P681R, and D950N. Spike decorates the surface of the pseudovirus, facilitating entry into cells expressing ACE2. BacMam is high titer and more cost effective to use than lentivirus.
Depending on your cell type of interest, additional proteins like ACE2, TMPRSS2, Neuropilin, or Cathepsin-L may need to be expressed to allow for pseudo SARS-CoV-2 entry. Viruses pseudotyped with the original spike protein and D614G spike protein are also available.
Materials in the Kit:
• Pseudo SARS-CoV-2 Delta Variant Green reporter
• Sodium Butyrate, 500 mM in H2O
Additional information
| Protocol for Use | https://montanamolecular.com/fluorescent-pseudo-sars-cov-2-reporter-protocol/ |
|---|---|
| Shipping | We strive for prompt delivery of each order, so we ship by FedEx 2-day service in lightweight, sturdy, and reusable/biodegradable packaging with an insulated cooler and moisture-resistant refrigerant gel packs. Shipping days are Mon-Wed so product does not sit in transit over the weekend. Web store ships to US & Canada only, contact sales for other locations. |
| MSDS | |
| More Info | https://montanamolecular.com/covid-19-reagents/pseudo-sars-cov-2-pseudovirus/ |
| Kit Volume | 5mL, 30mL, 60mL |
COVID-19 tools Publications
Pseudovirus and Host Factor Publications
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- Z. Wang, et al. Inhalation of ACE2-expressing lung exosomes provides prophylactic protection against SARS-CoV-2. nature communications. March 2024.
- F. Chen, et al. Hypercapnia increases ACE2 expression and pseudo-SARS-CoV-2 entry in bronchial epithelial cells by augmenting cellular cholesterol. Frontiers in Immunology. October 2023.
- Y. Kyosei, et al. Removal of soluble ACE2 in VeroE6 cells by 17β-estradiol reduces SARS-CoV-2 infectivity. Biological and Pharmaceutical Bulletin. October 2023.
- V. Fuochi, et al. Antiviral Efficacy of Heparan Sulfate and Enoxaparin Sodium against SARS-CoV-2: An In-Vitro/in-Silico Model. Preprints. October 2023.
- S. Shahbaz, et al. Analysis of SARS-CoV-2 isolates, namely the Wuhan strain, Delta variant, and Omicron variant, identifies differential immune profiles. Microbiology Spectrum. September 2023.
- M. Farrag, et al. Structural requirements of Holothuria floridana fucosylated chondroitin sulfate oligosaccharides in anti-SARS-CoV-2 and anticoagulant activities. PLoS One. May 2023.
A. Maurya, et al. Structure, anti-SARS-CoV-2, and anticoagulant effects of two sulfated galactans from the red alga Botryocladia occidentalis. International Journal of Biological Macromolecules. March 2023. - X. Mei, et al. An inhaled bioadhesive hydrogel to shield non-human primates from SARS-CoV-2 infection. Nature Materials. February 2023.
- M. Spampinato, et al. Effects of Mangiferin on LPS-Induced Inflammation and SARS-CoV-2 Viral Adsorption in Human Lung Cells. Pharmaceutics. December 2022.
- S. Huang, et al. Long Chain N3-PUFA Decreases ACE2 Protein Levels and Prevents SARS-CoV-2 Cell Entry. International Journal of Molecular Sciences. November 2022.
- S. Shahbaz, et al. Differential effects of age, sex and dexamethasone therapy on ACE2/TMPRSS2 expression and susceptibility to SARS-CoV-2 infection. Frontiers in Immunology. November 2022.
- R. Dwivedi, et al. Anti-SARS-CoV-2 and anticoagulant properties of Pentacta Pygmaea fucosylated chondroitin sulfate depend on high molecular weight structures. Glycobiology. September 2022.
- A. Puhl, et al. Vandetanib Blocks the Cytokine Storm in SARS-CoV-2 Infected Mice. ACS Omega. August 2022.
- S.T. Chuang, P. Buchwald. Broad-Spectrum Small-Molecule Inhibitors of the SARS-CoV-2 Spike-ACE2 Protein-Protein Interaction from a Chemical Space of Privileged Protein Binders. Pharmaceuticals. August 2022.
- B.J. Travis, et al. Significance of chlorine-dioxide based oral rinses in preventing SARS-CoV-2 cell entry. Oral Diseases. July 2022.
- R. Dwivedi, et al. Inhibition of SARS-CoV-2 wild-type (Wuhan-Hu-1) and Delta (B.1.617.2) strains by marine sulfated glycans. Glycobiology. July 2022.
- Z. Wang, et al. Exosomes decorated with a recombinant SARS-CoV-2 receptor-binding domain as an inhalable COVID-19 vaccine. Nature Biomedical Engineering. July 2022.
- BR Jo, et al. A Novel Antiviral Protein Derived from Oenanthe javanica: Type I Interferon-Dependent Antiviral Signaling and Its Pharmacological Potential. biomolecules. June 2022.
- S. Chuang, et al. Methylene Blue Is a Non-Specific Protein-Protein Interaction Inhibitor with Potential for Repurposing as Antiviral for COVID-19. Pharmaceuticals. May 2022.
- L. Souza-Moreira, et al. Poly(I:C) Enhances Mesenchymal Stem Cell Control of Myeloid Cells from COVID-19 Patients. iScience. April 2022.
- A. Puhl, et al. Vandetanib Reduces Inflammatory Cytokines and Ameliorates COVID-19 in Infected Mice. bioRxiv. December 2021.
- R. Dwivedi, et al. Structural and kinetic analyses of holothurian sulfated glycans suggest potential treatment for SARS-CoV-2 infection. Journal of Biological Chemistry. September 2021.
- Q. Zhang, et al. ACE2 interaction with cytoplasmic PDZ protein enhances SARS-CoV-2 invasion. iScience. July 2021.
- D. Bojadzic, et al. Small-Molecule Inhibitors of the Coronavirus Spike: ACE2 Protein-Protein Interaction as Blockers of Viral Attachment and Entry for SARS-CoV-2. ACS Infectious Diseases. May 2021.
- A. Riad, et al. Differential gene expression by RNA-Seq in Sigma-2 Receptor/TMEM97 knockout cells reveals its role in complement activation and SARS-CoV-2 viral uptake. bioRxiv. March 2021.
- D. Bojadzic, et al. Methylene Blue Inhibits the SARS-CoV-2 Spike-ACE2 Protein-Protein Interaction-a Mechanism that can Contribute to its Antiviral Activity Against COVID-19. Frontiers in Pharmacology. January 2021.
- Pramanik, et al. The rapid diagnosis and effective inhibition of coronavirus using spike antibody attached gold nanoparticles. Nanoscale Advances. January 2021.
3CLglow Publications
- J. Pérez-Vargas, et al. A novel class of broad-spectrum active-site-directed 3C-like protease inhibitors with nanomolar antiviral activity against highly immune-evasive SARS-CoV-2 Omicron subvariants. Emerging Microbes & Infections. August 2023.
- A. Citarella, et al. SARS-CoV-2 Mpro: A Potential Target for Peptidomimetics and Small-Molecule Inhibitors. biomolecules. September 2021.
