Robust Gi assay that, in real time, directly reports Gi mediated reductions in cAMP, across a variety of receptors and cell types on standard fluorescence plate readers or imaging systems.
- Simple, No cell lysis, No FRET, Single channel
- Can be targeted to specific cells in mixed cultures or subcellular organelles.
- Detection in living cells on fluorescent plate readers and imaging systems
- Robust Z′ statistics and consistent EC50 responses