Robust Gi assay that, in real time, directly reports Gi mediated reductions in cAMP, across a variety of receptors and cell types on standard fluorescence plate readers or imaging systems.

  • Simple, No cell lysis, No FRET, Single channel
  • Can be targeted to specific cells in mixed cultures or subcellular organelles.
  • Detection in living cells on fluorescent plate readers and imaging systems
  • Robust Z′ statistics and consistent EC50 responses

SLAS Discovery Article: Assay for Detecting Gαi-Mediated Decreases in cAMP in Living Cells

cAMP Assay Kit Details