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New AKAP-targeted cADDis Sensors

Most cAMP measurements are global measurements of total cellular cAMP. However when biosensors are targeted to different subcellular domains, they often reveal more nuanced views of very localized cAMP signaling. There are many different AKAP Proteins that are localized to different regions of the cell. These AKAP Proteins bind the regulatory subunits of the PKA enzyme, but they often interact with adenyl cyclases, phosphodiesterases, and PKA substrates. This has led to the idea that they define “signalsomes” in the cell. To measure cAMP changes that occur in these singalsomes we have begun to fuse the cADDis cAMP biosensor to AKAP proteins.

AKAP cADDis assay Beta-test signup:

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Prototype sensors available now:

Recent studies describing spatially limited cAMP gradientscAMP signaling poolsRAINs and signalsomes inspired us here at Montana Molecular. We created a new series of cADDis biosensors, enabling reliable measurements of cAMP signaling kinetics in various subcellular domains. These new cAMP assays are available for beta testing until March 1st.

Suggest the next AKAP/cADDis fusion sensor for development

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GPCR Biology

Increase your understanding of drug effects and GPCR biology with bright fluorescent assays for Gs, Gi, and Gq signaling in living cells.